Abstract
The characteristic symptom of the staphylococcal infection is the infiltration of polymorphnuclear leucocytes in the inflamatory area. Menkin reported the permeability factor (i.e. leucotaxine) which was extracted from the exsudate of inflamation. But the new substance which promotes the leucocytic migration is discovered in St. aureus or in the culture filtrate of St. aueus.
In this paper the assay method of L.P.S. (the substance which promotes leucocytes migration) was described.
Intracutaneous injection of St. aueus was followed by the skin resection, and the rcsected skin was crushed. The extracted leucocytes from crushed skin were counted by the ordinary method.
Another method for the determination of L.P.S. was adopted. The subcutaneous air cyste was followed by the injection of the fractions of St. aueusr. The conditions for the assay of L.P.S. were desecribed.
