Abstract
Transformation of paper mulberry plants (Broussonetia Kazinoki Sieb.) by infection with Agrobacterium tumefaciens was studied. Plants selected on kanamycincontaning medium were confirmed to express introduced foreign genes. The introduced foreign gene was the 35S-GUS gene obtained by fusion consisted of the CaMV35S promoter with the β-glucuronidase (GUS) gene, using the kanamycin resistant gene in a binary vector PBI121. Adventitious buds formed from infected tissues were selected on the MS medium containing kanamycin at 25-250mg/l. Adventitious buds were not formed on the medium containing kanamycin at higher concentrations than 100mg/l. The number of adventitious buds formed from the leaf discs and the cotyledons was counted at 40 days after the infection. Kanamycin resistant plants were obtained at a late of 28.4% from the leaf discs and 11.4% from the cotyledons, when they were transferred to the selection medium at 4 days after Agrobacterium infection. The GUS activity was detected in 12 plants among 13 kanamycin-resistant plants. Transformation was confirmed in 5 of the 7 plants with kanamycin resistance by Southern blotting.
