口腔病学会雑誌
Online ISSN : 1884-5185
Print ISSN : 0300-9149
Original Article
Gnetin-C, a Resveratrol Dimer, Inhibits the Growth and Virulence of Porphyromonas gingivalis
YOKOSE MakoKOMATSU KeijiSHIBA TakahikoTANAKA DaikiTAKEUCHI YasuoIKEDA Yuichi
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2024 年 91 巻 1 号 p. 47-65

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  Gnetin-C is a resveratrol dimer that is reported to have various health-promoting effects, including anti-inflammatory, anti-oxidant, and anti-cancer effects. Gnetin-C promoted alveolar bone healing in periodontal tissues in animal experiments. However, its effects on bacteria have not been investigated. In this study, we compared the effects of gnetin-C with those of monomeric resveratrol and Melinjo seed extract, the source of gnetin-C, on the periodontopathic bacterium Porphyromonas gingivalis (P. gingivalis) and examined its mechanism of action. The results illustrated that gnetin-C strongly inhibited bacterial growth, bacterial cell adhesion, and biofilm binding compared to monomeric resveratrol. Melinjo seed extract displayed greater effectiveness than monomeric resveratrol but was less effective than gnetin-C treatment. In addition, cell surface irregularities and cell membrane ruptures were observed after gnetin-C treatment by scanning electron microscopy, suggesting that bactericidal effect by gnetin-C.

  RNA sequencing analysis and quantitative polymerase chain reaction revealed marked changes in the gene expression profiles of P. gingivalis after gnetin-C treatment. In particular, the expression of genes related to fimbriae, iron metabolism, proteases, and amino acid and vitamin metabolic pathways was significantly suppressed, whereas that of oxidative stress-related genes was significantly increased. Reactive oxygen species levels were significantly decreased by gnetin-C treatment in P. gingivalis. These results indicate that gnetin-C inhibits the growth and virulence of P. gingivalis. This study demonstrates the potential of gnetin-C in the treatment and prevention of periodontal disease. Gnetin-C can be applicable as a self-medication agent for prevention or treatment of periodontal diseases.

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© 2024 The Stomatological Society, Japan
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