The structure and characters of the transparent layer and adjacent layers of dentin of extracted human third molars having decay was investigated by microscopy, Knoop hardness determination, optical microscopy, microradiography and scanning and transmission electron microscopy, obtaining the findings below:
1. On the vertical sections of decayed dentin, the normal layer, the lightly transparent layer, the heavily transparent layer and the discolored layer were distinguished.
2. Deposition of platelike crystals on the dentinal tubule lumen walls was found starting from the uppermost part of the normal layer and filling up many of the tubules in the lightly transparent layer.
3. In the heavily transparent layer, most dentinal tubules were filled up with rhombohedral crystals resulting remarkable increase of X-ray opacity in the layer.
4. Knoop hardness was highest at the junction between the normal and lightly transparent layers and to some extent lower at the heavily transparent layer showing crystal decrease of intratubular layer and intertubular dentin due to carious lesion.