顎・顔面・口腔外科領域において使用される医用高分子材料の生体為害性に関する研究

―培養細胞に対する溶出物質の毒性ならびに溶出傾向の検索―

抄録

This study concerns the research conducted on the biocompatibility of medical synthetic polymers utilized in the field of maxillo-facial surgery, such as Realastic®, Epithane-3®, HC-107/ON-105 system and Silastic® MDX4-4210.
Three methods were used to evaluate the cytotoxicity to the L cell on each of these four polymers to investigate their biocompatibility. These three methods were 1) cell nucleus count, 2) morphological observation using a phase contrast microscope and 3) investigation of DNA, RNA and protein synthesis by the incorportion rate of radioactivity using 3H-thymidine, 3H-Uridine and 3H-Leucine.
Results:
Polmyer Cell nucleus count Morphology DNA, RNA and protein synthesis
REALASTIC® Cytostatic in 1-10mg/ ml
Cytostatic in 1-3.2mg/ml Cytocidal in 10mg/ml
DNA, RNA and protein inhibited but RNA inhibition was moderate.
EPITHANE-3®
Cytostatic in 1mg/ml Cytocidal in 3.2-10mg/ml
Cytostatic in 1mg/ml Cytocidal in 3.2-10mg/ml
DNA, RNA and protein inhibited.
HC-107/ON-105 system
Cytostatic in 10-100mg/ml
Cytostatic in10-100mg/ml
DNA, RNA and protein inhibited.
SILASTIC® MDX4-4210
Non-cytotoxicity in 10-100mg/ml
Non-cytotoxici- DNA and RNA inhibited moderatety in 10-100mg ly but protein accelerated./ml
Elution Tendency;
By repeated extraction five times during 24 hours using a medium containing 10% serum, the decrease in the DNA synthesis inhibition of the L cell was measured and the following results were obtained: Realastic®; 100% Epithane-3®; 860, HC-107/ON-105 system; 36% and Silastic® MDX4-4210; 76% .