マウス顔面形成におけるアクチンの役割に関する研究
1988 年 55 巻 4 号 p. 615-638
詳細
1988 年 55 巻 4 号 p. 615-638
Mammalian facial development involves formation of nasal placode (NP), its invagination and protrusion of facial prominences, and consequently fusion of them. These processes have some similarities to neurulation and other organogeneses, in which contraction of actin filaments at the apical site has been shown to be possible morphogenetic force. The purpose of this study is to examine the role of actin filaments in developing nasal epithelium (NE) .
C57BL/6 mouse embryos sacrificed on 8.5 to 11.5 days of gestation (plug day=0) were fixed in 4% PFA in PBS. 57, um of frozen sections were stained with rhodamine-phalloidin (R-P) . Before formation of NP, there was no specific localization of F-actin. When NP was formed, F-actin was observed at the apical site of the placode and also of the invaginating NE. On the contrary, it was localized at the basal site of the epithelium of developing nasal protrusions.
Mouse embryos were exposed to 0.15g/ml of cytochalasin D (CD) for 2 hours in vitro using whole embryo culture system. Facial malformation was observed in 70.40 of the CD-treated embryos at the end point of the culture for 24 hours. The distribution of F-actin in NE was observed to be partially broken in R-P staining image just after the treatment.
These findings suggested that F-actin in NE may play a critical role in mouse facial formation.
