ウシ骨基質由来骨吸収因子の部分精製とその性質に関する研究

抄録

The mechanism of bone remodeling has been investigated by many researchers. However, little is known about the role of the local factors which exist in the bone matrix.
In this study, the author attempted to purify the bone-resorbing factor from the bovine bone matrix and to find its role in bone remodeling.
The demineralized bovine bone matrix was extracted with 4M Gdn-HC1 and the boneresorbing factor was purified by means of heparin affinity and gel filtration chromatography. The analysis of bone resorption was carried out by the measurement of the released ⁴⁵Ca from the pre-labeled mouse calvariae.
The results of this study were that the bone-resorbing factor in the bone matrix was suggested to be a heat-stable glycoprotein having a molecular size higher than 150, 000, which stimulated osteoclastic bone resorption via a prostaglandin-mediated mechanism.