Abstract
A study has been made for the purpose of methodological aspect on Tamm-Horsfall glycoprotein, glycosaminoglycans and glycopeptide contents in such urinary fractions as the one the filtered and the other the supernatant following centrifugation. The contents were measured both biochemically and immunologically. The filtration procedure greatly reduced the Tamm-Horsfall glycoprotein content, while the centrifugation had little effect on its content. Furthermore, either filtration procedure or centrifugation had no effect on the any of uronic acid, hexose or sialic acid content of the glycosaminoglycan-glycopeptide preparations.