1971 年 2 巻 2 号 p. 73-84
Anti-tumor effect of extracts (Bamfolin) from bamboo grass on patient diagnosed cancer was at first found clinically. At the fundamental studies on this, many investigators have been presented evidence which suggests that antitumor effect might due to indirect effect of the polysaccharide against cancer through the host. We, recently, found that the extracts contained the other components affected the tumor cells directly. Crude Bamfolin was fractionated in the order of molecular weight by dialysis against distilled water and gelfiltration on Sephadex G-25 and G-50. Each fractions were tested by means of CAP-method, contact test and experimental therapeutic test using Ehrlich carcinoma and Sarcoma-180. From these tests, we found that the sugar fraction (mol. wt. >10, 000) showed the inhibition of the growth of Ehrlich ascites carcinoma by i.p. injection in mice and the sugar fraction (mol. wt. <10, 000) had only slight anti-tumor effect. The first injection of the test samples was started at 6 hr after tumor implantation and the following injections were done twice a day for three days from next day. Then, in 1mg/mouse×7 doses, the sugar fraction (mol. wt. >10, 000) saved all mice. But the sugar fraction (mol. wt. <10, 000) saved only about 20-40 per cent of mice. From these data, it is suggested that the action may be directly cytocidal. The details of differences between this effective sugar fraction and former polysaccharide, which is so-called host mediator, are under investigation.