1977 Volume 23 Pages 39-41
As a result of inhibition by pisatin to radial mycelial growth of each fungus cultured on medium the median effective dose (ED 50) values for fungi were as follows: 44ppm for Cladosporium fulvum non-pathogenic to Pisum sativuin, 60 and 62 ppm for Botrytis cincrea and Rhizoctonia solani polyxenic to many plants, respectively, and more than 68 ppm for Ascochyta pisi and Fusasium solani f. pisi pathogenic to P. sativuan. Acquired tolerance to pisatin was slightly obtained in A. pisi, B. cinerea and C. fulvum by successive cultures of these fungi for seven or ten generations at intervals of several days on media containing 100ppm of pisatin.