2019 Volume 31 Issue 4 Pages 158-162
A novel core-shell ion-exchange filler composed of an ion-exchange porous shell layer formed on a hard polymer core was prepared for application to HPLC. We examined the effect of the thick shell of ion-exchange filler St-80 controlled by the weight ratio of the monomer for the core and shell (20:80) on the retention time and the theoretical plate number (N) in the separation of saccharides. A mixed aqueous sample of inositol, glucose, fructose and sucrose was well separated under alkaline conditions (100 and 150 mmol/L NaOH) at flow rates of 0.3-0.9 mL/min. The St-80 filler provides good chromatographic separation of saccharides, a good degree of separation between glucose and fructose and a high number of theoretical plates for saccharides.
