The Review of Laser Engineering
Online ISSN : 1349-6603
Print ISSN : 0387-0200
ISSN-L : 0387-0200
Imaging Membrane Potential Dynamics in Neurons
Mutsuo NURIYA
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JOURNAL FREE ACCESS

2012 Volume 40 Issue 4 Pages 224-

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Abstract

Neurons perform fast information processing in the form of membrane potential dynamics. Therefore, detailed examinations of membrane potential dynamics in neurons are crucial to the understanding of neuronal information processing and brain functions. However, attempts to characterize membrane potential dynamics in neurons have been hampered by the small size and the complexity of neurites, such as dendritic spines and axons, where conventional electrophysiological techniques have limited access. Furthermore, while optical imaging techniques provide better access to these structures, fluorescence-based imaging techniques are not suitable for quantitative membrane potential measurements. Second harmonic generation (SHG) imaging, which can overcome these problems, can be utilized for quantitative membrane potential imaging. Here, I review the basic principles and applications of SHG imaging in neuroscience researches and discuss the potential of this technique.

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© 2012 by The Laser Society of Japan
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