Binding of Tobacco Mosaic Virus to Membrane Material Isolated from Tobacco Leaves

Abstract

Binding of tobacco mosaic virus (TMV) to disrupted tobacco leaf membrane was studied. Mem-brane isolated from tobacco leaves was treated successively with (NH₄)₂SO₄, Li-diiodosalicylate and then pronase. TMV-binding substance was thus isolated in a soluble form. From enzymatic digestion experiments, it was suggested that the binding substance was composed of lipid and carbohydrate.