MICROBIOLOGY and IMMUNOLOGY
Online ISSN : 1348-0421
Print ISSN : 0385-5600
ISSN-L : 0385-5600
Sequence Analysis of the actA Gene of Listeria monocytogenes Isolated from Human
Kohji MoriishiMichinori TeraoMinako KouraSatoshi Inoue
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1998 Volume 42 Issue 2 Pages 129-132

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Abstract
The region encoding proline-rich units of actA genes was amplified from 24 strains of Listeria monocytogenes using polymerase chain reaction (PCR). PCR products of 13 strains showed the expected size of 623bp, whereas those of 11 strains showed a short size of 518bp. The shortening of these PCR products resulted from the deletion of one proline-rich unit. These results indicate that ActA proteins are divided into at least two different types which are unrelated to bacterial serotypes.
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