質量分析のためのサンプル調製（タンパク質ネットワーク大規模解析）

抄録

Functional proteomics aims to determine the cellular functions of genes directly at the protein level, and includes the comprehensive analysis of protein-protein interactions by affinity-based isolation of protein complexes. Mass spectrometry (MS) is a powerful method for the identification of proteins. To enhance the sensitivity and resolution of the MS/MS analysis, we developed a novel direct nanoflow liquid chromatography/MS/MS system. Furthermore, to enhance the sensitivity of the whole analysis system, we optimized the conditions of sample preparation. In this review, we describe the pull-down procedures.