Online ISSN : 1347-5320
Print ISSN : 1345-9678
ISSN-L : 1345-9678
Direct and Rapid Quantification of Calcium Phosphate Precipitate on Titanium by X-Ray Fluorescence Analysis Using Fundamental Parameter Method
Kozue SatohMasashi KomataManabu MizuhiraKazuaki WagatsumaNaofumi Ohtsu
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2013 Volume 54 Issue 5 Pages 817-824


In the present study, we propose a characterization technique to determine the amount of calcium phosphate (CP) precipitate formed on a titanium (Ti) substrate. The quantitative analysis of the CP precipitate on a metallic substrate is significant for researchers of metallic biomaterials because CP that spontaneously precipitates in a simulated-body fluid gives information on the bioactivity of the metallic biomaterials. We focused on X-ray fluorescence (XRF) analysis and adopted the thin-film fundamental parameter method (thin-film FP method) because it allows direct (non-pretreatment) and rapid quantitative analysis without any reference materials. We show that XRF analysis using the thin-film FP method can be adequately applied to the quantitative analysis of the CP precipitate in the simulated-body fluid immersion test. We also show that the density of the CP-precipitate layer can be estimated by combining the XRF results with images of cross-sectional scanning electron microscopy (SEM).
Consequently, XRF analysis combined with the thin-film FP method can provide a convenient means to evaluate the CP precipitate on a Ti substrate, which improves the accuracy and accessibility of the simulated-body fluid immersion test.

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© 2013 The Japan Institute of Metals and Materials
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