MATERIALS TRANSACTIONS
Online ISSN : 1347-5320
Print ISSN : 1345-9678
Directional Control of Mature Osteoblast Derived from Juvenile Mouse Calvariae
Ryosuke OzasaAira MatsugakiYoshihiro IsobeTaro SakuTakayoshi Nakano
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2017 Volume 58 Issue 6 Pages 958-962

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Abstract

Bone tissue has a highly anisotropic microstructure comprised of biological apatite (BAp) and collagen fibrils, which is closely related to bone mechanical function. The formation of anisotropic bone microstructure is governed by bone-forming osteoblasts; therefore, isolation of functional osteoblasts and regulation of their arrangement for generating bone tissue with optimally-oriented microstructure during bone reconstruction are important. In this study, we established the isolation and culture conditions of mature osteoblasts derived from juvenile mice (2-week-old). Osteoblasts from juvenile mice expressed significantly higher level of osteoblastic markers (alkaline phosphatase, osterix, and osteocalcin) than osteoblasts from neonatal mice, indicating that juvenile osteoblasts are promising materials for bone tissue engineering. Moreover, alignment of the mature osteoblasts was successfully regulated using oriented collagen substrates. This is the report that shows directional control of mature osteoblasts isolated from juvenile mice.

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© 2017 The Japan Institute of Metals and Materials
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