MATERIALS TRANSACTIONS
Online ISSN : 1347-5320
Print ISSN : 1345-9678
ISSN-L : 1345-9678
Enhanced Cell Proliferation on Biomedical Titanium Surfaces by Laser Ablation-Induced Micro- and Nanoscale Hybrid Structures
Hwa-Teng LeeChing-Chi Lin
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2019 年 60 巻 9 号 p. 1799-1806

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The cell proliferation performance of pure titanium substrates was enhanced by modifying the surface morphology using an ultraviolet laser with a wavelength of 355 nm and travel speeds ranging from 10∼300 mm/sec. Rat calvarial osteoblast cells were cultured on the sample surfaces for 1∼7 days. The cell proliferation was investigated via 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays. Scanning electron microscopy observations showed that the laser ablation (LA) surfaces had a hybrid micro- and nanoscale structure consisting of microscale grooves with nanoscale agglomerations on their surface. For a low laser travel speed of 10 mm/sec, the grooves had a width of approximately 5.44∼10.03 µm. For the maximum travel speed of 300 mm/sec, the grooves reduced in height, but increased in width to around 10.97∼20.06 µm. The agglomerations on the grooves had a size of around 30∼100 nm; with larger agglomerations being formed at a lower laser travel speed. The XRD analysis results revealed the presence of titanium compounds (TiO and TiN0.3) on the LA surfaces ablated at lower travel speeds of 10 mm/sec and 50 mm/sec, respectively. The MTT measurements showed that the LA samples yielded a better cell proliferation rate than a sandblasted acid-etched titanium sample or a machined titanium sample. Furthermore, the cell proliferation rate increased with a decreasing laser travel speed. In general, the present results confirm the feasibility of laser ablation surface modification as a means of promoting the cell proliferation rate on titanium bioimplants.

The proliferation performance of pure titanium substrates was enhanced by modifying the surface morphology using an ultraviolet laser with a wavelength of 355 nm and travel speeds ranging from 10∼300 mm/sec. Rat calvarial osteoblast cells were cultured on the sample surfaces for 1∼7 days. The cell proliferation was investigated MTT assays. SEM observations showed that the laser ablation surfaces had a hybrid micro- and nanoscale structure consisting of microscale grooves with nanoscale agglomerations on their surface. The MTT measurements showed that the LA samples yielded a better cell proliferation rate than a sandblasted acid-etched titanium sample or a machined sample. Furthermore, the cell proliferation rate increased with a decreasing laser travel speed. In general, the present results confirm that surface modification via laser ablation treatment provides an effective means of promoting the cell proliferation of titanium bioimplants. Fullsize Image
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© 2019 The Japan Institute of Metals and Materials
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