Non-human MHC genotyping methods using next generation sequencing

Abstract

Genotyping methods for non-human MHC by using next generation sequencing (NGS) require different approaches from the HLA genotyping, because the structure of non-human MHC genes is quite different from the human MHC and lack of sufficient reference information. In this review, we will introduce representative NGS-based MHC genotyping methods for non-human species, focusing on data analyses of sequence data obtained for non-human MHC.