Medical Mycology Journal
Online ISSN : 2186-165X
Print ISSN : 2185-6486
ISSN-L : 2185-6486
Short Report
Simple Modified Method for Fungal Slide Preparation
Shigeru Fujita
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2013 Volume 54 Issue 2 Pages 141-146

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Abstract
  Slide culture preparations are necessary for accurately identifying dermatophytes. Because the standard slide culture technique is difficult for dermatologists to carry out, there is a need for an economical and easy-to-prepare system without special equipment. We placed a block of agar medium on a square cover glass (24 × 24mm) in the bottom of a case made of polypropylene (Free case, PK-201, HINODEWASHI CO., LTD. Japan). The agar block inoculated with fungi was overlaid with another cover glass on top of it. A plastic petri dish containing 4 cases as described above was sealed with adhesive plastic tape and incubated at 27°C.
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© 2013 The Japanese Society for Medical Mycology
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