Medical Mycology Journal
Original Article
Preparation and Biological Characterization of Limulus Factor G-activating Substance of Aspergillus spp.
Yuichiro KuroneKen-ichi IshibashiDaisuke YamanakaNoriko N. MiuraYoshiyuki AdachiNaohito Ohno
著者情報
ジャーナル フリー

58 巻 (2017) 4 号 p. E121-E129

詳細
PDFをダウンロード (467K) 発行機関連絡先
抄録

Aspergillus is a medically important fungal genus that causes a life-threatening infection known as aspergillosis in immunocompromised patients. β-1,3-Glucan is detected in the plasma of patients with aspergillosis and appears to be useful for the diagnosis of aspergillosis. In this study, we cultured Aspergillus spp. in a chemically defined liquid medium and prepared an Aspergillus water-soluble fraction (ASWS) from the culture supernatants. ASWS was found to be primarily composed of polysaccharides and proteins. Nuclear magnetic resonance analysis suggested that ASWS is a complex carbohydrate, consisting of α-1,3-glucan, β-1,3-glucan, galactomannan, and protein. The ASWS from Aspergillus fumigatus showed limulus factor G activity, whereas zymolyase-treated ASWS did not. ASWS was eliminated from the blood more rapidly than Aspergillus solubilized cell wall β-glucan. We analyzed the reactivity of human immunoglobulin towards ASWS by an enzyme-linked immunosorbent assay. Anti-ASWS antibodies were detected in human sera, with titers differing among individuals. This study demonstrated that the ASWS corresponds to the limulus factor G-activating substance found in the blood of patients with aspergillosis.

著者関連情報
© 2017 日本医真菌学会
次の記事

閲覧履歴
feedback
Top