Microvascular Reviews and Communications
Online ISSN : 1880-5906
Print ISSN : 2188-1707
ISSN-L : 2188-1707
EXPLORE THE ANGIOGENESIS OF AUTOLOGOUS TRANSPLANTED BRAIN TISSUES IN RABBITS
Jin Xuelong
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JOURNAL FREE ACCESS

2014 Volume 7 Issue 1 Pages 34

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Abstract

Objective: This study intends to discuss about the methodology of rabbit’s brain tissue transplantation, including the way of operation and the law of microcirculatory formation.

Methods: 20 male Japanese white rabbits (1.2˜1.3 kg) were chosen for an intracerebral transplantation test, which were anaesthetized by 3% pentobarbital sodium in vein before receiving an intracerebral transplantation operation. A window was opened on their parietal bone and the cortical brain tissues on the symmetrical areas on the left and right side of the rabbits’ parietal cortex areas were exchanged and transplanted. Gentamycin sulfate was injected each day to resist infection. Ten and twenty days later, an observation was made as to the survival of the transplanted area and host brain tissue. A microcirculation color camera system was used to analyze the pictures of angiogenesis. With regard to the survival of transplanted brain tissues, their changes in micromorphology were observed. Besides, pathological sections were also prepared to determine their surviving conditions on a cell level.

Result:
(1) Surgical operation has contributed to a satisfactory morphological anastomosis between transplanted brain tissues and host brain tissues.
(2) Analysis of the pathological sections of the transplanted brain tissues showed traces of surviving neural cell.

Conclusions: Under the given conditions, transplanted brain tissues can maintain neuron’s survival, and can be nourished by angiogenesis and characteristic microcirculation connections with host brain tissues.

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© 2014 by Japanese Society for Microcirculation
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