Determination of ochratoxin A in foodstuffs by monoclonal antibody affinity chromatography

M. NAKAJIMA; H. TERADA; K. HISADA; H. TSUBOUCHI; K. YAMAMOTO; Y. ITOH; T. UDA; O. KAWAMURA; Y. UENO

doi:10.2520/myco1975.1990.32_50

M. NAKAJIMA, H. TERADA, K. HISADA, H. TSUBOUCHI, K. YAMAMOTO, Y. ITOH, T. UDA, O. KAWAMURA, Y. UENO

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JOURNAL FREE ACCESS

1990 Volume 1990 Issue 32 Pages 50-51

DOI https://doi.org/10.2520/myco1975.1990.32_50

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Abstract

A rapid and 1-step clean-up method for determination of ochratoxin A (OTA) was developed by anti OTA monoclonal antibody affinity chromatography. This method offered good recovery and significantly cleaner HPLC chromatograms than conventional approaches. This affinity column was regenerated for many times with no apparent loss of activity. The detection limit of OTA was 0.5 ppb.

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