Development of enzyme sorbent immunoassay for rubratoxin B

Abstract

A polyclonal antibody for Rubratoxin B (RB) was raised in rabbit and the competitive direct ELISA method to detect RB was set. Optimum concentration of antibody and enzyme conjugate were 1:1000 and 1:1500, respectively. Fifty % inhibition for RB at these conditions were about 5 ng/ml. This ELISA system can be used up to 50% methanol or 20% acetonitrile for solvent of sample solution. Aflatoxins (B₁, B₂, G₁ and G₂), cyclopiazonic acid, kojic acid, patulin, ochratoxin A, sterigmatocystin and zearalenone did not inhibit this reaction. However, unknown interferences from various agricultural commodities were observed and further more study is needed to apply this method to real samples.