Studies on Urinary Amino-Acids by Paper Partition Chromatography

Abstract

Amino-acids in urine were analyzed by means of paper partition chromatograph, both clinically and experimentally.
Usually two-dimension method is performed, but it is also of some clinical value to detect amino-acids with large Rf by the use of one dimension method.
Alanine, Glycine and Taurine always appear in urine, no matter whether it be normal or pathological.
As a rule, amino-acids detected in urine of hepatic patients increase in number; especially in the jaundice-stage of acute hepatitis there can be ascertained several sorts of amino-acids, while in the reconvalescence they decrease in number showing tendency to become normal.
In cases with liver cirrhosis, detected amino-acids are of various number, only the appearance of amino-acids with large Rf is more remarkable than in those who suffer from hepatitis. This occurrence is neither concerned in existence of icterus nor ascites.
In cases with hepatic coma, some increase in number very much, others not so markedly. That is to say, patients suffering from hepatic coma do not always excrete high-degree amino-aciduria.
Number of amino-acids proved in hepatic cancer is relatively few, and there is no great difference between in cancer and innormal.
Number of amino-acids detected in cholecystitis and cholecystopathy fluctuates instablely. In above mentioned cases there can not be proved any special amino-acid appearing without fail.
Attitude of amino-acids in urine of rats which livers were damaged with various kinds of toxins, is similar to that of clinical cases.
High protein diet after administration of hepatic toxins is admitted as effective to prevent the appearance of a certain amino-acids in urine.