ON THE COMPENSATORY EXCRETION OF ELECTROLYTES FROM THE LIVER IN CASES OF RENAL DISTURBANCES

REPORT 3 THE APPLICATION OF CATTLE BILE

Abstract

Normal rabbits were set up for artificial bile fistula just after the bilateral nephrectomy, and then the choleletic effects of Sodium dehydrocholate (abbreviated as “DHC”) were observed. The dose of DHC injected per kg of body weight was 1.0ml of 20 per cent aqueous solution. Whereas the effect of DHC injection was very notable at first, but at reinjection after three hours such a choleletic effect was no more seen.
In order to prevent the decline of its effect at reinjection, cattle cystic bile, twice diluted with physiological saline solution, was, introduced into the intestine, but no improvement was obtain. Also the dried cattle bile on sale, which was diluted with 20 per cent glucose solution to 1 per cent solution, turned harmful.
Alcoholic extract of the dried cattle bile remained still harmful and two out of three rabbits were sacrificed.
On the contrary, the ether extract obtained from the residue after the alcoholic abstraction restored the choleletic effect of the DHC reinjection perfectly.
Thus introducing the ether soluble ingredient of cattle bile into the intestine of rabbits, the outflow of electrolytes (Cl, Na) became also notable.