SERUM TRANSAMINASE ACTIVITIES
(3rd Report: CLINICAL AND EXPERIMENTAL STUDIES)
1958 Volume 47 Issue 8 Pages 994-1007
Details
1958 Volume 47 Issue 8 Pages 994-1007
The author's colorimetric assay method of serum transaminase (T) and its modification for tissue T were used in these studies. The results were indicated as γ of oxalacetic acid or pyruvic acid, which was formed after the incubation time under definite conditions.
RESULTS
A. Clinical Studies:
1. Normal values of serum T were as follows:
The mean of serum glutamic-oxalacetic transaminase (SGOT) was 8.10±2.15γ (180 cases) and that of serum glutamic-pyruvic transaminase (SGPT) was 4.10±1.63 γ (120 cases).
2. SGOT was elevated but SGPT was unchanged in a case of acute myocardial infarction. In various kinds of liver diseases, especially in acute hepatitis, both were markedly elevated.
3. The clinical course and examination data of six representative cases were described and briefly discussed.
4. The difference of mobility between SGOT and SGPT was clinically and experimentally established from paper-electrophoretical studies.
B. Experimental Studies:
1. Not only an increase of serum T activities but a decrease of liver T activities was found in CC14 treated mice. With these findings and the histological examination of the liver, it was confirmed that serum T activities was a good indicator of acute liver damage.
2. Localization of T in cellular fractions of liver homogenate was studied by Schneider's method. The decrease of T activities was most remarkable in the mitochondrial fraction, in CCI4 treated rabbits.
3. The increase of serum T activities was compared with the decrease of liver T activities in CC14 treated rabbits. In this study, total amount of increased serum T activities was calculated from the serum T activities per 0.1cc and the circulating plasma volume which was determined by radioactive phosphorus method. The ratio of increased SGOT to decreased liver GOT was 17.3% and it was 41% in GPT. Dr. Wroblewski's presentation, in which SGPT was clinically more useful than in SGOT in the detection of acute liver damage, was experimentally confirmed with these data.
