Nano Biomedicine
Online ISSN : 2185-4734
Print ISSN : 1883-5198
ISSN-L : 1883-5198
ORIGINAL ARTICLES
Montipora digitata Exoskeleton Derived Aragonite Particles are useful Scaffold for Tissue-engineered Vascular Graft in Vitro
Tomoharu OKAMURANaoya UEMURAShunsuke BABANaoko YASUDAHideyuki YAMASHIROKoichi IMAITetsunari NISHIKAWAHideo SHIMIZUMuneyasu SHIDAKazuya TOMINAGAAkio TANAKA
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2017 Volume 9 Issue 2 Pages 105-111

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Abstract

Designing a preoperative tissue engineering vascular graft (TEVG) that is immunologically compatible with the host is one of the goals of vascular tissue engineering. Angiogenesis is an important early step in wound healing, and the three-dimensional formation of capillaries using scaffold contributes greatly to the success of bone regeneration therapy. In this study, aragonite particles derived from exoskeleton of Montipora digitata (a kind of hard coral) as a scaffold of TEVG were applied to three-dimensional culture of normal human dermal skin fibroblasts and normal human umbilical cord vein endothelial cells, and the effect was observed morphologically. As a result, the particles promoted cell proliferation and capillary differentiation three-dimensionally in vitro. Also, incorporation of calcium from the aragonite particles into co-cultured normal human dermal skin fibroblasts and normal human umbilical vein endothelial cells was observed. Thus, aragonite particles were inferred to be a useful scaffold for 3 dimensional capillary culture.

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© 2017 Nano Biomedical Society
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