2006 年 29 巻 1 号 p. 20-26
Recent experimental studies have demonstrated that oxygen free radicals have important roles of various neuronal conditions. Especially, neuroemergency diseases such as neurotrauma are strongly related with oxidative stress. However, there are few studies proved the existence of free radicals in human because of difficulty in measurement. In this study we introduced free radical (alkoxyl radical) monitoring in neurotrauma. Blood samples were collected from the catheter of the internal jugular bulb. The alkoxyl radical level was measured extra vivo by ESR spectrometry using 5, 5-dimethyl-1-pyrroline-1-oxide (DMPO) as a spin trap, which was obtained from Dojin Chemical (Tokyo, Japan). Electron spin response detection of the spin adduct was performed at room temperature using a JESREIX X-band spectrometer (JEOL, Tokyo, Japan).
Alkoxyl radical levels of all patients were elevated in comparison with control. Alkoxyl radical level was suppressed after administration of edaravone (30 mg i.v). Brain hypothermia treatment suppressed alkoxyl radical in the injured brain while conversely causing systemic oxidative stress formation. Alkoxyl radical levels in patients treated with hyperbaric oxygen therapy (HBO) were elevated within treatment rapidly.
This method is useful to monitor the free radical level immediately at bedside and it will enable to detect the oxidative stress and the severity of brain damage.
