Abstract
A chromatographic elution pattern, an absorption spectrum or a NMR spectrum is obtained generally as an overlapping curve. Resolution of the overlapping curve into component curves would give some useful informations.
A derivative of logarithm of a Gaussian curve gives a linear function. The gradient and x-intercept of the liner function indicates a standard deviation and a center of the Gaussian function, respectively. Based on this Bhattacharya's method, we made a cemputer program for the curve analysis including an X-Y plotter.
An application of the program to elution patterns of THO, in gel chromatography, proved correspondence of a calculated curve with observed curves. Further application to an acidic protein, a basic protein or some proteins which might have characteristic interaction with gel, also proved the good fitness of the method.
From these results, any elution pattern of gel chromatography could be analyzed into the component Gaussian curves, when the conditions were kept proper.
