1972 年 34 巻 4 号 p. 428-434
In order to detect the drugs causing patients to suffered from drug eruptions peripheral blood lymphocytes taken from the patients were cultured with the suspicious drugs for 120 hours. Culture rods were divited into three groups:
1. positive control group with phytohaemagglutinin
2. negative control group without any drugs
3. main group with some stepped concentration of suspicious drugs.
Cells after 120 hours incubation were morphologically classified into three types and counted; large cell, intermediate cell and small cell. The results were reached by using the ratio of these three cell types. Simultaneously, incorporation of tritiated thymidine was also examined by autoradiography and Backman’s liquid scintillation counter.