Forty-seven tumors of bone and soft tissues were investigated histochemically, among which 11 osteogenic sarcomas and 13 giant cell tumors of bone were contained.
Specimens from frozen section and touch smears were prepared for 4 special stains of PAS, Sudan III, alkaline and acid phosphatase. For the detection of alkaline and acid phosphatase, simultaneous azocoupling method of Tomonaga et al., was employed because of its excellent stainability.
Interesting results observed were as follows.
1) In case of giant cell tumor of bone, high activity of acid phosphatase was observed both in stromal cells and in tumor giant cells.
2) Moderate activity of alkaline phosphatase was seen in both cells in 5 cases.
3) One case of Ewing sarcoma showed the reaction of alkaline phosphatase, which was ordinary reported to be abscent.
4) The enzymatic reaction was low in many tumor of soft tissues.
