実験腫瘍による Acridine Orange と Argon Laser を用いた Photodynamic therapy

抄録

We use Donryu rats (100g) transplanted Walker's Carcino-sarcoma subcutaneously as experimental tumors.
When tumor attained 2cm of its maximal diameter, we injected 3mg Acridine orange intraperitoneally.
After 48^hr, Argon-Laser was irradiated (5w, 250sec, C. W) on this tumor, and still more on second day from irradiation, we made tumor cells separatedly and stained them by Feulgen method.
We measured DNA-content distribution with MMSP-RF, Absorption spectrum with MMSP-TU, and emission spectrum with MMSP-RFF.
Still more we checked transtimidal change of intratumor temparature during Argon-Laser irradiation with Thermography & Thermister.
As to DNA content distribution, there was no discrepancy between control group (administrated nothing or Acridine-Orange only) and photodynamic therapy group (Acridine-Orange+Argon Laser).
As to emission spectrum of intracellular Acridine-Orange, orthochromasic cell has maximal emission peak at 515nm, but metachromasic cell has at 535nm or more longer wave shift.
As to emission spectrum of Feulgen stained cells, normal DNA has maximal peak at 610nm, but Acridine-Orange administrated & photodynamic therapy group have maximal peak at 620nm or more longer wave lenght.
Shift to longer wave length can be seen also in heat-degenerated DNA.
During photodynamic therapy, the highest temparature retained less than 40°C.
Adding to these results, photodynamic-therapied Rats could survive more longer than any other therapied rats significantly.
And we could observe perfect disappearance of transplanted tumors in some rats.
From these observations, we think Photodynamic-Therapy is one of the useful anticancer-therapy.