抄録
The uptake of carboxyfluorescein diacetate (CFDA) into live cells was used as the basis for a simple, rapid and fully automated micromethod for determination of cell growth. The aim of the investigation was to adapt the CFDA method for assessing the cytotoxicity of materials for use in medical devices. Thus, the cytotoxicity of biomaterials could be detected and quantitated at the same level of sensitivity as with the conventional [3H] thymidine incorporation. The CFDA method can prove to be rapid, reliable and well suited for several applications involving limiting numbers of cells and biological reagents.
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