A Rapid Method for Detection of Cytotoxicity of Metal using Carboxyfluorescein: Second Report

Abstract

A cytotoxicity test of biomaterials (nickel, chromium, and iron) using carboxyfluorescein diacetate (CFDA) was performed with rat fetal lung fibroblast (RFL-Fb). Cytotoxicity of chromium and nickel on viability of RFL-Fb was severe and the number of viable cells was decreased dose-dependently. However, cytotoxicity of iron on RFL-Fb was not detected. This method might be reliable to detect cytotoxicity of biomaterials on various kinds of cells.