1964 年 61 巻 4 号 p. 221-229
The author tried to investigate the chemical analysis of bile using the infrared absorption spectroscopy to elucidate its component. The biliary specimen, taken by the percutaneous cholangiographic procedure pre-operatively and also during surgery, was lyophilized and examined. The results of 141 cases of cholecystic bile and 18 cases of hepatic bile were proved as follows:
1) The total lyophilized bile was classified into 3 patterns according to its spectroscopic findings.
2) In the type I, it is characterestic that Lecithin Bile Salts System (LBS system), Chloroform fraction by Isaksson's method, occupies to a high extent and extremely coincides with the infrared absorption of the LBS system.
3) In the type II and III, it is characteristic that the bile salts extracted by ethanol and sediment extracted by neither ether, chloroform nor ethanol show the highest ratio and extremely coincide with the infrared absorption respectively.
4) Among the bile, the only type I shows the absorption of 1740cm-1 which is almost due to Lecithin.
Experimentally, the infrared absorption spectroscopies were done for the cholecystic bile of normal dogs.
1) The infrared absorption spectrum shows similar pattern which may be due to taurocholate and Lecithin without any body difference.
2) By ligation of cystic and common bile duct, the absorption of 1740cm-1 decreased.