腸内細菌によるTyramineの産生について

抄録

Amines produced from amino acid by enterobacteria are not sufficiently detoxicated in affected liver to manifest various infavourable effect. Amines are produced by amino acid decarboxylase of bacteria in digestive canal. In this investigation, tyrosine decarboxylase activity and tyramine production of various enterobacteria were especially studied. Tyrosine decarboxylase activity was determined by manometric method and tyramine was proved by paperchromatography or as the crystal.
1) Tyramine production from tyrosine was examined for 1004 strains of enterobacteria isolated from feces of 84 individuals including healthy persons and patients with some digestive diseases. Gram negative bacilli producing tyramine were proved only in a low percentage, among them, kiebsiella was highest in percentage, followed by proteus and pseudomonas, then by escherichia. However majority of gram positive cocci, including all of St. faecalis tested, produced tyramine.
2) There was no distinction between healthy persons and patients as to tyramine producing enterobacteria. It was thought that production of tyramineby enterobacteria would be enhanced in the case of coexistence of particular strain and tyrosine in high content in digestive canal.
3) Tyrosol, p-hydroxyphenyllactic acid, p-hydroxyphenylpropionic acid and p-hydroxyphenylacetic acid were proved as the decomposition products of tyrosine with both tyramine producing and non-producing strains.
4) Intensity of tyramine production by enterobacteria was estimated by the Warburg manometric method in adition to paper chromatographical identification and chemical isolation as crystal. Data of the three comparative investigation kept a considerble parallelism.