Nippon Shokakibyo Gakkai Zasshi
Online ISSN : 1349-7693
Print ISSN : 0446-6586
Histochemical Studies on Human Stomach
Part II on Dehydrogenases
Takao KAWASHIMAShigeru TAKEMOTOHiroshi YOSHIDANobuto SHIMATANIMasato NAKAMURAAkira NISHIYAMAKisaburo TAKEUCHIHideo NOBUTOTakashi SEITOTakuro OGATA
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1965 Volume 62 Issue 12 Pages 1641-1646_2

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Abstract

Hydrolytic enzymes on human stomach had been described histochemically in part I.
Therefore a histochemical study on dehydrogenases was performed in the present paper, in which succinic, lactic, malic, α-glycerophosphate, glutamic, β-hydroxybutyric, glucose-6-phosphate and isocitric dehydrogenases were examined.
Materials were obtained by the same methods in part I.
Specimens were cut at 20 micra in -20°C cryostat, then the sections were stained by the methods described by Pearse with nitro-BT as the electron acceptor.
For the stainings of SDH and NAD linked dehydrogenases, superficial epithelium of pyloricgland area was not the same of fundcorpus gland area enzymatically, and the former was stronger reactive in basal side than in surface side of pyloric area. Thestaining reaction was heavy in parietal cells, but weak or moderate in neck cells, zymagemic cells and pyloric gland cells.
Strong staining intensity of NADPlinked dehydrogenases was noticed in gastric superficial epithelium, while weakly or hardly stained in gland cells. These eight dehydrogenase activities were kept or slightly increased in earlier stage of necrosis and degeneration and aparrently decreased in necrotic or degenerative region.
Atrophic mucosa showed decreased reaction and hyperplastic or hypertrophic regions showed a tendency to increased the activty. Staining reaction in intestinal metaplasia. was more intense than in normal mucosa and similar to that in duodenal villi.

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© The Japanese Society of Gastroenterology
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