膵外分泌機能に関する研究

第一報Pancreozymin-secredn試験並びにsecrctin試験の正常値に関する検討

抄録

This preliminary report deals with the derivation of technics for pancreozymin-secretin test and secretin test described by Sun et al. and Dreiling et al. as the exocrine function of the pancreas, and the results obtained from 40 patients without pancreatic diseases on whom such derived tests were performed.
1) The technic of pancreozymin-secretin test derived and currently employed may be summarized as follows. Pancreozymin (Boots), in a dose of 1 unit per kg of body weight, was given first intravenously and duodenal content was collected for two 10-minute period postpancreozymin followed by secretin (Boots), in a dose of 1 unit per kg of body weight, collecting duodenal content for two 10-minute period followed by two 20-minute period in total 60 minutes postsecretin.
In a case of secretin test was duodenal content collected after administration of secretin in a dose of 1 unit per kg of body weight for two 10-minute period followed by two 20-minute period in total 60 minutes postsecretin.
Continuous aspiration with a suction pump at a negative pressure of 250 mm of water was maintained through the cllection of duodenal content.
The volume, amylase activity and bicarbonate concentration of the duodenal content collected were determined respectively. Blood sample was taken before pancreozymin or secretin was injected and at 1, 2, and 4 hours after secretin and serum amylase activity was determined.
2) Analysis of duodenal content after pancreozymin-secretin administration consists amylase output per kg of body weight 20- minute period after pancreozymin stimulation, volume output per kg of body weight, amylase output per kg of body weight, maximal amylase concentration, maximal bicarbonate concentration, and bicarbonate output per kg of body weight for 60-munute period after secretin stimulation.
Secretin test, on the other hand, consists measurement of volume output per kg of body weight, amylase output per kg of body weight, maximal amylase concentration, maximal bicarbonate concentration, and bicarbonate output per kg of body weight.
“Norms” of pancreozymin -secretin test and secretin test have been established by statistical analysis of the above mentioned measurement of duodenal content.
3) Serum amylase response to pancreozymin-secretin test or secretin test was considered positive when serum amylase value valied more than 35 Somogyi units after pancreozymin or secretin stimulation.
4) The results of duodenal contents in postsecretin 60-minute period of “normal” subjects in pancreozymin-secretin test did not differ from those normal values obtained after secretin alone.