コロイドによる網内系 (RES) 填塞時のトランスアミナーゼ

アルカリ性フオスフアターゼの動態

抄録

Littoral cells constitute a considerable mass of the liver but their function has not been separated from that of the hepatocytes. In view of the possibility that the earliest changes might involve the former type of cells in diseases like viral hepatitis, the present study was carried out to elucicate the role the reticuloendothelialsystem in alterations of certain enzymes of the serum and tissue. Rats and rabbits were used and they were given intravenously either Thorotrast, india ink or glucan and serum and tissue GOT, GPT and alkaline phosphatase were determined in comparison with the control. Furthermore, histological studies after colloid administration, of the liver and spleen, as well as determinations of these enzymes in separated Kupffer cells were carried out. Kupffer cells were separated from hepatocytes by forced uptake of carbonyl iron colloid and magnetic collection of iron particle containing cells.
The results indicated that serum GOT, GPT and aklaline phosphatase levels increased shortly after colloid administration and all colloids tested had more or less similar effects. Separated Kupffer cells were shown to contain considerable amounts of these enzymes as expressed per unit of nitrogen. These results seem to suggest that early changes in serum enzymes in certain diseases might reflect some alterations of the littoral cells of the liver.