肝傷害時の電解質代謝異常に関する研究 (第1報)

カリウム欠乏食飼育ラツトにおけるカリウム保持現象, および四塩化炭素肝傷害によるその障害

抄録

Potassium conservation, i.e. progressive decrease in the excretion of potassium into the urine during potassium-deficient feeding, was observed in normal and chronic CCl₄ intoxicated rats, and found to be impaired in the latter.
Fourty-eight male rats, weighing 170 to 270g, were devided into the placebo control and CCl₄ treated group, to which 0.1ml per 100g of olive oil or 20% CCl₄, respectively, was injected subcutaneously twice a week for three weeks. In this period rats were kept on standard diet (K:0.30mEq/g). Thereafter they were kept on mild (K:0.089mEq/g) or strict (K: 0.0056mEq/g) potassium-deficient diet for 3 to 10 days. Five hours' urine after previous electrolyte loading was colected before and after potassium-deficient feeding. Blood and muscle samples were taken at the end of potassium-deficient feeding. Water, K, Na, Cl and creatinine in these materials were measured.
1) In the CCl₄ treated rats kept on standard diet, urinary elimination of K after electrolyte loading was larger, that of Na was smaller and urinary K/Na ratio was higher than those in the control rats.
2) In the control rats kept on potassium-deficient diet, urinary elimination of K decreased (p<0.01), that of Na temporarily decreased and then increased (p<0.05), and the K/Na ratio fell remarkably (p<0.01).
3) In the CCl₄ treated rats kept on strict potassium-deficient diet, the urinary eimination of K decreased and K/Na ratio fell as well as in the control rats. In the CCl₄ treated rats kept on mild potassium-deficient diet, however, potassium concervation was impaired, i.e. the urinary elimination of K on the 3rd day and K/Na ratio on the 10th day were significantly greater than those in the control rats.(p<0.05).
4) In both control and CCl₄ treated rats serum K level decreased and Na level increased after 10 days of potassium-deficient feeding. However, these changes were not statistically significant.
5) Water content of muscle was unaffected by both CCl4treatment and potassium-deficient feeding. But K content of muscle in the CCl4 treated rats was smaller and Na content was larger than those in the control rats. K/Na ratio in muscle in CCl₄ treated rats was lower than that in the control rats (p<0.05).
6) Serum level and urinary output of creatinine were greater in the CCl₄ treated rats, but without any significant change increatinine clearance.
The present results may show the usefulness of these experimental conditions in the investigation on electrolyte abnormalities in liver damage.