血清アルカリ・フォスファターゼに関する研究

第2報閉塞性黄疸時における血清アルカリ・フォスファターゼ上昇機転の実験的研究

抄録

To elecidate the mechanism of serum alkaline phosphatase elevation in obstructive jaundice, the author has investigated activities of intrahepatic alkaline phosphatase, serum total alkaline phosphatase, and 2 fractions of the serum enzyme by disc-phoresis in rats after the ligation of biliary ducts with or wihtout resection of the small intestines. The activity of intrahepatic alkaline phosphatase was found in the wall of sinusoids and Glisson's sheaths. There was no significant difference in the activity in sinusoidal wall between the control group and the treated groups; a strong stainability was demonstrated in Glisson's sheaths in the group of biliary duct ligation after 4 days. The activity of total serum alkaline phosphatase in treated groups was elevated. In the group with biliary duct ligation alone, the activity reached its maximum on the 3rd day, reducing rapidly thereafter to the lowest on the 7th day, then tended to increase again. In the group with biliary duct ligation with small intestine resection, the activity was obviously lower than the other treated group, and reelevation of the activity was not observed.
The stainability of the 2nd bands of the enzyme obtained by Disc-phoresis was characterized by an initial increase of fast band after treatment followed by a reduction associated with a gradual increase of slow band in the group of biliary duct ligation. In the group with biliary duct ligation and small intestine resection, there was a moderate initial posttreatment increase with a gradual reduction thereafter; there was little increase in the intensity of slow band.
From the foregoing data it was concluded that the elevation of the activity of serum alkaline phosphatase in obstructive jaundice in rats can be divided into initial and latter phases, and that small intestines are playing some role in the latter elevation of the enzyme in Glisson's sheaths of the liver.