胆細管にかんする病理組織学的研究

第二篇肝硬変にみられる胆細管の組織化学的研究

抄録

For the purpose to elucidate the relationship between bile ductule and liver cell in chronic hepatitis, cholangiolitic hepatitis, and liver cirrhosis was studied by histopathological and histochemical observations and also by the reconstruction model of thehuman liver biopsy specimens. The results of the study are briefly presented in the following.
1. It was elucidated that bile ductule cell contain a substance positive to PAS stain, and the enzymatic activity of the cells in the proxymal portion was more intense than those of the distal portion. The difference is due to the number of the mitochondria visualized with the Regand stain, and has a parallel relation to the destruction of liver parenchyma. In the threedimensional observation with reconstruction model of the liver, there is an ample reason to suppose polarization from theproliferated bile ductule cells into liver cell through the overall changes observable such as the communication of bile ductules with regenerated nodules. However, it has been impossible to determine the direction of the polarization.
2. In the sections of livers prepared with cryostat the activity of aminepeptidase is detected to a moderate degree in the cytoplasm of liver cells but the bile ductules show hardly any enzymic activity.
3. The proliferated bile ductules in cholangiolitic hepatitis presented somewhat different histological and histochemical properties from those observed in either chronic hepatitis and liver cirrhosis.
4. Cluster of cells with high enzymic activity were observed around the regnerated nodules in an array as if to encapsulate them, and it is assumed that this picture reflects the condensation of mitochondria due to the comprission of liver cells by regenerated nodules