ヒト胃粘膜の組織化学的研究

各種脱水素酵素について

抄録

In order to investigate the correlation of morphological and functional changes of the gastric mucosa, the histochemical studies of human gastric mucosa with special reference to various dehydrogenases were performed. The enzymes studied were succinate dehydrogenase (SDH), lactate dehydrogenase (LDH), glutamate dehydrogenase (GDH) and malate dehydrogenase (MDH).
Methods
The biopsies of gastric mucosa were obtained from certain five areas of the antrum and body by gastrocamera-gastric biopsy method under fluoroscopy. The histochemical studies of the mucosa were done. SDH was examined by Nichlas's method. LDH, GDH and MDH were examined by modification of the Nachlas and Seligman method. Nitro-BT was used in all of their examinations.
Results
1) SDH activity.
The parietal cells (PC) showed positive SDH activity. The activity of the PC was decreased when atropnic changes of the mucosa was severe. The surface epithel (SE) usually showed negative activity. When the mucosa has severe atrophic change, especially metaplasic gastritis, the activity was positive in the SE, and duodenal mucosa showed also positive in the SE.
2) LDH
The PC showed positive LDH activity. When atrophic changes of the mucosa was severe, the activity of the PC was decreased as the SDH activity. Most of the SE showed positive and the muscularis mucosae also showed positive.
3) GDH
GDH activity was positive in the PC. But the activity was weaker than SDH or LDH activity. GDH activity was also positive in the SE but it was also weaker than the LDH activity.
4) MDH
MDH activity was positive in the PC. But the activity was much weaker than the GDH activity. No obvious relation between atrophic changes of the mucosa and the MDH activity in the PC was observed. The MDH activity in the SE was positive in a half same as the GDH activity.
5) Correlation of gastric acidity and the SDH activity of the PC.
The SDH activity was strongly positive in the PC of the stomach with hyperacidity. On the other hand, the activity was decreased in the PC of the stomach with hypoacidity. It is apparent that gastric acidity is about parallel with the SDH activity of the PC.