実験的肝傷害白鼡における肝内短絡血流測定法による測定

D-galactose-1-¹⁴Cによる測定

抄録

A D-galactose-1-¹⁴C solution was injected into the superior mesenteric vein or the hepatic artery of the rats. Ten seconds after the injection, the hepatic artery and the portal vein were ligated, and the liver was resected. Per cent intrahepatic shunt was calculated from the radioactivity of the resected liver and that of the injected solution added to the liver with same kind of lesion.
When the solution was injected into the mesenteric vein, per cent intrahepatic shunt of rats with fatty liver produced by feeding of choline deficient diet and rats with acute carbon tetrachloride intoxication was almost the same as the normal rats. Rats with hepatic fibrosis showed slight increase, and rats with hepatic cirrhosis marked increase in per cent intrahepatic shunt. There was no significant difference of per cent intrahepatic shunt between rats with hepatic carcinoma produced by feeding of 3'-methyl-DAB and normal rats.
When the solution was injected into the hepatic artery, per cent intrahepatic shunt of rats with normal liver was higher than that of the mesenteric venous injection. The difference of per cent intrahepatic shunts in rats with hepatic cirrhosis between the mesenteric venous injection and the hepatic arterial injection was lesser than that in normal rats.
Per cent intrahepatic shunt of rats with hepatic carcinoma increased markedly when the solution was injected into the hepatic artery. This can be explained by the facts that the cancerous tissue is supplied by the hepatic artery and the intrahepatic shunted blood flow in the cancerous tissue is not measured by the method of intra-portal injection but of intra-arterialinjection. Increase of per cent intrahepatic shunt in rats with hepatic carcinoma is ascribed to abnormal galactose metabolism in tumor tissue rather than to appearance of the hepatic arterio-venous anastomoses. Abnormal metabolism of galactose in hepatic carcinoma produced by feeding of 3'-methyl-DAB was shown in an experiment: ¹⁴CO₂ production from D-galactose-1-¹⁴C in tumor slices decreased markedly as compared with that in the control liver.