胃•十二指腸潰瘍の酵素組織化学的研究

抄録

I have studied histochemical changes with eight different hydrolytic and oxidative enzymes in 31 gastric ulcers, 11 duodenal ulcers, 11 experimental gastric ulcers of rats and 70 gastric and duodenal mucosa with other various gastric diseases.
1) Increased activity of various enzyemes were observed in the granulation tissues under the ulcers of stomach and duodenum. Alkaline phosphatase (A.lP) showed marked activity, while Lactic dehydrogenase (LDH), DPN diaphorase (DPNd) showed moderate activity and Acid phosphatase (Ac. P), Adenosine triphosphatase (ATPase), Aminopeptidase (Amp) showed slight activity. Esterase (Est), Succinic dehydrogenase (SDH) showed very little activity. Experimental ulcers of rats showed the same results.
2) Fibfoblasts, collagen fibers and capillary walls in the granulation tissue under the ulcers showed marked activity of Al. P, especially in the young granulation tissue. Healing scar tissue showed little activity. The fact suggests that Al. P is related to the healing of ulcers.
3) The enzymes including Al. P, LDH, DPNd, Ac. P, ATPase and Amp showed increased activity in the granulation tissue, while they showed decreased activity in the scar tissue. I concluded that metabolism was very active in the healing granulation tissue.
4) Capillary walls in the granulation tissue showed marked activity of Al. P and ATPase. These enzymes may be related to the transport of the material through the capillary walls.
5) Histiocytes in and around the necrotic layer were plump and showed marked activity of Al. P and Est. They may be related to the cleaning of the base of ulcers.
6) Normal gastric mucosa did not show any activity of Al. P and Amp, while mucosa showing intestinal metaplasia showed marked activity of these enzymes. In addition to these enzymes, mucosa with intestinal metaplasia showed marked activity of the other enzymes and showed the same distribution of enzyme activity as in duodenal mucosa.