Studies on nucleie acids and nucleoproteins in carcinoma tissues

the infrared spectra of nucleic acids and nucleoproteins extracted from malignant tumors

Abstract

In order to study the difference in the molecular structure of nucleic acids and nucleoproteins of carcinoma tissues from those of normal tissues, infrared spectra of these substances were analysed. The nucleic acids and nucleoproteins were extracted from the carcinoma tissues and normal tissues of gastrointestinal tracts in the human body, AH 130 ascites tumors, DAB hepatomas and the liver of normal rats. The infrared spectra of these extract-substances were determined by using KBr tablets at 18°C and 40% relative humidity.
It was not possible to differentiate DNA of normal tissues from that of malignant tumors by means of infrared spectra, but possible to differentiate DNA of human tissues from that of rat tissues. Comparing DNP of the normal tissues with that of malignant tumors, the absorption bands in the 1700 to 1300cm-¹ region in the human body, in the 1750 to 1400cm-¹ region in AH 130 ascites tumors, in the 1600 to 1400cm-¹ region in DAB hepatomas were differed from each other. For RNA the intensity of the absorption bands were differed in the 1550 to 1350cm-¹ region and in the 995cm-¹ band in the liver of normal rats and AH 130 ascites tumors. With DAB feeding, 1525cm-¹ band of RNA of DAB hepatomas has gradually increased in intensity, whereas 1485cm-¹ band has gradually decreased. Comparing RNP of the normal tissues with that of malignant tumors, the absorption bands in the 1650 to 1250 cm-¹ region in the human body, in intensity of 1455cm-¹ band and 1385cm-¹ band in AH 130 ascites tumors, in the 1600 to 1300cm-¹ region in DAB hepatomas were differed from each other.