ガスクロマトグラフィーによる血中尿中および臓器内単糖類の定量
特に Xylitol を中心として
1969 年 66 巻 9 号 p. 962-971
詳細
1969 年 66 巻 9 号 p. 962-971
A method for quantitative analysis of monosacharide and their polyols in serum, urine and liver by gas chromatography is described. These studies have been carried out with a Yanagimpto GCG-5DH with flame ionization detector using SE-30 column as liquid phase at 170°C.
Analytical procedures were as follows: serum was deproteinized by the method of Somogyi and the supernatant was dried completely using rotary evaporator at 60°C. The residue was dissolved in anhydrus pyridine, and then trimethylsilylation was done adding hexamethyldisilazane and trimethylchlorosilane prior to injection to gas chromatograph. Urine, if not albuminuria, was dried without any treatment and trimethylsilylation was performed. Liver was homogenized in physiological saline with sodium fluoride and also deproteinized by the method of Somogyi. Following procedures were as same as serum. Sorbitol was used as internal standard according to the stability of its trimethylsilyl ether and appearing its peak between α-and β-glucose. Peak areas of glucose and xylitol obtained by gas chromatography showed to be proportional to their weights, but it was necessary to multiply the value obtained by gas chromatography by the coefficiency (0.92) for getting the weight of xylitol.
Blood glucose levels determined by gas chromatography and auto analyzer method showed a satisfactory agreement. The average value determined by gas chromatography was 6.9% lower than that obtained by auto analyzer method.
The anlytical examples of xylitol tolerance test to patients with liver disease and with diabetes mellitus were presented as the clinical application.
This method is not also sensitive, but also accurate because non-sugar substances which give reducing reaction can not be determined. Therefore, this is a definitely exellent method for separation and determination of monosacharides and their polyols in blood, urine and various tissues, and is also available for detecting disorders of carbohydrate metabolism in clinical and experimental studies.
