Experimental studies on the effect of sodium hippurate upon the biliary excretion of sulfobromophthalein

Abstract

Rat experiments were undertaken to examine the alterations of steps involved in the transfer of BSP from blood to bile after the intravenous administration of sodium hippurate.
1) Following a single intravenous administration of BSP, biliary excretion of BSP was increased by hippurate.
2) Distribution of BSP after a constant infusion of BSP was estimated. Through the intravenous administration of hippurate, a distinct elevation of the maximal transport of BSP into bile (BSP-Tm), a significant increase of unconjugated BSP storage in the liver (BSP-S) and a moderate degree of choleresis were observed.
3) Through an addition of hippurate a significant acceleration of BSP-glutathione conjugating enzyme activity was observed on liver homogenate but no acceleration was noted on liver slices.
4) Through an addition of hippurate a slight degree of inhibition of BSP-binding affinity of serum albumin was observed.
5) It was concluded that an increased biliary excretion of BSP by sodium hippurate was resulted from a significant elevation of BSP-Tm and a slight degree of inhibition of BSP-binding affinity of serum albumin.