Histochemical Studies on the Experimental Ulcer Induced by the Clamping-Cortisone Method in Rats

Report I: Clamping and Clamping-Cortisone Groups

Abstract

In this paper, the results obtained by histochemical studies on the clamping-cortisone ulcer in rats are reported. The clamping-cortisone ulcer was made by the procedures mentioned before. In the histochemical studies, PAS, Alcian-blue, TBM. Aldehyde Fuchsin and neutral red staining methods were used for examining Polysaccharides changes in the tissue. Further, Gomori's and Azo staining methods were used for studing alkaline phosphatase. The animals were divided into two groups, i.e. the group treated by Clamping alone (the control group) and the group treated by the Clamping-Cortisone method. The PAS staining of the mucous content in the regenerative mucosa and the connective tissues of the base of the ulcer developed earlier and was stronger in the control group compared with that of the Clamping-Cortisone group. At the base of the ulcer, a clear difference of the staining in the middle and base layer was observed between the control group and the Clamping-Cortisone group. The degree of staining with PAS in the mucous membrane in the non-ulcerous region was also stronger in the control group compared with that in the Clamping-Cortisone group. In the Alcian-blue staining, the same results were obtained, but Alcian-blue staining activity became lower in the latter stages of the ulcer. The fibroblast and Collagen fiber were not stained. The spaces between the Collagen fibers were stained strongly. The TBM staining (pH2.5) degree did not show any remarkable difference between the control group and the Clamping-Cortisone group. Staining activity of the Alkaline phosphatase in the regenerative mucosal cells and connective tissue in the base of the ulcer developed earlier and stronger in the control group compared with that of the C-C ulcer. From these findings one of the reasons of prolongation of the ulcer induced by the Clamping-Cortisone method seemed to be attributed to decrease of mucous secretion from the mucosal cells and of polysaccharides in the connective tissue. Furthermore, it is also classified that there is a close relationship between alkaline phosphatase activities and the healing of the ulcer.